Coding

Part:BBa_K801071:Design

Designed by: Roman Prechtl   Group: iGEM12_TU_Munich   (2012-09-21)

7-methylxanthine N-methyltransferase CaMXMT1-strep


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 976
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Synthesized by IDT, Yeast codon optimized
Keywords: koffein, coffeine, caffeine, coffee, xanthosine, coffea, arabica


Abbreviations: CaMXMT1 = coffea arabica 7-methylxanthine N-methyltransferase 1

Design Notes

Related BioBrick: BBa_K801074 BBa_K801077

Quality control measures:

  • test digest has been performed with the use of Xba1 and Pst1 (see mainpage)
  • part was totally sequenced

Backbone:

  • Backbone name: pSB1C3'

Protein coding:

  • CaMXMT1

Enzymatic activity:

EC-number [http://enzyme.expasy.org/EC/2.1.1.160 2.1.1.160]

Cytotoxicity:
cytotoxic for S.cervisiaegrowth inhibition at 10 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • synthesized by IDT


Organism:

  • Genesequence derived from coffea arabica
  • codonoptimized for saccharomyces cerevisiae
  • designed for saccharomyces cerevisiae


References

Literature references:

  • [http://www.ncbi.nlm.nih.gov/pubmed/12746542 Pubmed: Uefuji H., Ogita S., Yamaguchi Y., Koizumi N. and Sano H., 2003: Molecular cloning and functional characterization of three distinct n-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants.]
  • [http://www.ncbi.nlm.nih.gov/pubmed/16247553 Pubmed: Uefuji H., Ogita S., Yamaguchi Y., Koizumi N. and Sano H., 2005: Caffeine production in tobacco plants by simultaneous expression of three coffee N-methyltrasferases and its potential as a pest repellant.]
  • [http://www.ncbi.nlm.nih.gov/pubmed/16925551 Pubmed: Kuranda K, Leberre V, Sokol S, Palamarczyk G, François J., 2006: Investigating the caffeine effects in the yeast Saccharomyces cerevisiae brings new insights into the connection between TOR, PKC and Ras/cAMP signalling pathways.]

Database references:

  • [http://www.ncbi.nlm.nih.gov/nuccore/AB048794.1 GenBank: Coffea arabica CaMXMT1 mRNA for 7-methylxanthine N-methyltransferase, complete cds]
  • [http://www.uniprot.org/uniprot/Q7FRH9 Uniprot: 7-methylxanthine N-methyl transferase]-->